Residual Bovine Serum Albumin (BSA) Determination by ELISA
We offer Residual BSA determination by ELISA. This assay is performed using the Bovine Albumin ELISA kit manufactured by Bethyl Laboratories. Maxisorp Nunc 96 well plates are coated with a sheep anti-BSA antibody. Samples and standards (400 ng/ml down to 0 ng/ml) are added to the appropriate wells and following incubations and several washes, the conjugate HRP-tagged antibody is added to all wells followed by a substrate. Optical density readings are reduced using a four parameter logistic in order to generate a standard curve and calculate sample residual BSA concentrations.
This is an example of a standard curve obtained in the Residual Bovine Serum Albumin ELISA Assay. The sample concentration is deduced from the four parameter logistic formula generated in the assay. Standard range is 0-400 ng/ml.
Sample requirements: 1 ml or one cell pellet shipped overnight on ice packs
Turnaround time: One week
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