Clongen Labs offer a wide range of Microbiology Testing Services. Routine culture, identification and antibiotic sensitivity determination is now offered on samples from the following sources (Please call the laboratory to inquire about the proper sample type, sample submission form, cost and sample collection requirements):
Click here to download sample submission form
In order to request a blood culture, please contact the laboratory and request the blood culture medium. A special blood culture medium will be shipped out to you (the patient or the clinic). Up to 10 ml of whole blood should be aseptically injected into the bottle (for children, 5 ml is adequate, for neonates, 1-2 ml is adequate). It is preferable that the patient gets their blood collected locally at our laboratory in Germantown, MD. Please call us first to make an appointment.
For best recovery of microorganisms, it is best to collect blood during fever or chills. Multiple blood collections may be indicated in some cases for better recovery. The patient should not be on antibiotics for at least 1 week and if the patient is taking antibiotics, that should be indicated on the sample submission form. If yeast is suspected, that should be indicated on the sample submission form so that we use special procedures for isolating and identifying yeast.
Turnaround time: 10-12 days
Cost: $225 (if antibiotics sensitivity is performed on any isolated organisms, there is an additional cost of $125)
Collection,transport and storage of specimen: Stool are the major source of specimen, vomit can be used.
- specimen should be collected within 48 hours of illness
- stools samples can be stored at 4°c for weeks if not tested immediately after collection.
Identification: Biochemical identification, Bacitracin disk for ß hemolytic Streptococci
Sensitivity test: Kirby Bauer Method
Culture from the maxillary, frontal or other sinus should be collected by the syringe aspiration technique and culture set up for recovery of both aerobic and anaerobic bacteria (call lab. for transport medium kit)
Collection of genital specimen from females:
Semen should be collected in a wide mouth sterile container and shipped immediately to laboratory for best culture results
From breast secretion or breast aspiration.
WOUNDS AND ABSCESSES:
Swab samples are not suitable for anaerobic culture
Blood for culture, CSF, Tissue, peripheral catheters, Indwelling central venous catheter, Implantable device. Please call lab. to request blood culture bottles prior to blood collection
SPECIMEN REJECTION CRITERIA:
Improper collection or transport
Poor labeling: Specimen without label or request form, prolonged transport, leaking container, (Anaerobic culture for a specimen transported aerobically), container not sterile, dry specimen, specimen received in formalin.
The following antibiotics are used for specific isolates
Gram positive Cocci :
Staphylococci: Penicillin, Oxacillin or Methicillin, Vancomycin, Clindamycin, Azithromycin or Erythromycin or Clarithromycin, Trimethoprim-Sulfamethoxazole, Gentamicin, Tetracycline, Rifampicin.
Enterococcus spp: Penicillin or Ampicillin, Vancomycine, Gentamicin ( high level resistance screen only),
Streptomycin (high level resistance screen only), Ciprofloxacine, Norfloxacin, Nitofurantoin and Tetracycline.
Streptococcus pneumoniae:Penicillin, Amoxicillin or Amoxicillin-Acid clavulanic, Erythromycin, Trimethoprim-Sulfamethoxazole, Ceftriaxone or cefotaxim, cefuroxim, Vancomycin, Tetracycline, Imipenem.
Other Streptococci spp: Penicillin or Ampicillin, Erythromycin, Vancomycin, Clindamycin, Cefotaxime or Ceftriaxone, Azithromycin or Clarithromycin, Ofloxacin.
Gram Negative Bacilli:
Enterobacteriaceae: Ampicillin, Cefazolin or Cephalotin, Amoxicillin/clavulanic acid, Cefoxitin, Cefotetan, Cefotaxime or Ceftriaxone, Gentamicin, Amikacin, Trimethoprime-Sulfamethoxazole, Tetracycline, Nitrofurantoine, ciprofloxacin, Norfloxacin, Ofloxacin.
Pseudomonas aeruginosa: Ticarcillin, Carbenicillin, Piperacillin, Ceftazidime, Ticarcillin/clavulanic acid,
Aztreonam, Imipenem, Amikacin, Gentamicin, Tobramicin, Ciprofloxacin.
Penicillin, Ceftriaxone, Cefuroxime, Tetracycline, Spectinomycin, Ciprofloxacin or Ofloxacin.
Ampicillin, Trimethoprime-Sulfamethoxazole, Amoxicillin/clavulanic acid, Azithromycin or Clarithromycin, Cefuroxime, Ceftriaxone, Cefotaxime, Tetracycline, Ciprofloxacin or Ofloxacin, Rifampicin.
Penicillin G, Metronidazole, Clindamycin, Imipenem, Cefoxitin, Ceftriaxone, Cefotaxime, Amoxicillin/clavulanic acid, Chloramphenicol, Piperacillin.
Direct examination: Direct wet preparation and concentration procedures will be performed
Giardia, Dientamoeba, Chilomastix, Enteromonas and Rotatomonas.
Pathogenic flagellates are: Giardia lamblia, Dientamoeba fragilis and Trichomonas vaginalis.
Transmission is via ingestion of food or water
Specimen is fresh stool
Direct examination: Wet mount and permanent stained smear of fecal material are the predominant specimen used to diagnose infections with flagellates.
Giardia lamblia: The diagnosis of Giardia is established by the microscopic examination of stool for the presence of cysts and or trophozoites.Nucleic Acid Detection Technique: We also offer Giardia lamblia detection by PCR.
The advantage of PCR is the increased sensitivity compared to direct examination
Around 100,000 named species of fungi. The more common encountred human mycoses are:
Deep Mycoses: Blastomycosis, Coccidiomycosis, Sporotrichsis
Opportunistic Mycoses: Aspergillosis, Candidosis, Geotrichosis
Subcutaneous Mycoses: Maduramycosis (Mycetoma), Sporotrichosis (Sporothrix schenkii).
Superficial Mycoses: Tinea versicolor, Dermatomycoses ( Dermtophytes), Tinea and Onychomycosis.
Common specimen collection sites for recovery of Clinically Significant Fungi:
Blood (Yeast), CSF (Cryptococcus neoformans), Sterile fluid, Bone marrow, KT IV, Ear external, Hair, Skin, Nails, Medical devices, Urine, Vaginal, Tissue, Prostatic fluid (Blastomycosis).
Swabs samples are not adequate for recovery of fungi.
Direct examination: It is highly recommended that a direct microscopic examination be made for fungal specimens. Hyphae, yeast formws small budding yeast will be reported
Skin scales, nail scraping and hair should be examined after potassium hydroxide (KOH 10 %) treatment.
Incubation: 30°c for 3 weeks.
Specimen collected for yeast (Vaginal and oropharyngeal) are incubated for1 week.