Southern Blot
Hybridization differs from PCR in that the target DNA is not amplified. The target sequence is detected using a signal amplification technology where a labeled probe hybridizes to the target and following stringent washing steps, the probe/target complex is detected using color development. Southern blotting requires digestion of the genomic DNA with restriction enzymes to produce fragments of various sizes. Those fragments are run on an agarose gel and blotted onto a membrane. The membrane is hybridized with a probe designed to complement the target DNA. Following development of the signal or image recording, the resulting banding pattern is analyzed to determine the genotype or carrier status of animals within the tested colony.
Western Blotting
We offer Western Blotting Services for any protein product. Please contact
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