We offer Residual BSA determination by ELISA. This assay is performed using the Bovine Albumin ELISA kit manufactured by Bethyl Laboratories. Maxisorp Nunc 96 well plates are coated with a sheep anti-BSA antibody. Samples and standards (400 ng/ml down to 0 ng/ml) are added to the appropriate wells and following incubations and several washes, the conjugate HRP-tagged antibody is added to all wells followed by a substrate. Optical density readings are reduced using a four parameter logistic in order to generate a standard curve and calculate sample residual BSA concentrations.

This is an example of a standard curve obtained in the Residual Bovine Serum Albumin ELISA Assay. The sample concentration is deduced from the four parameter logistic formula generated in the assay. Standard range is 0-400 ng/ml

Sample requirements: 1 ml or one cell pellet shipped overnight on ice packs

Turnaround time: One week