Hybridization differs from PCR in
that the target DNA is not amplified. The
target sequence is detected using
a signal amplification technology
where a labeled probe hybridizes to
the target and following stringent
washing steps, the probe/target complex
is detected using color development. Southern
blotting requires digestion of the
genomic DNA with restriction enzymes
to produce fragments of various sizes. Those fragments are run on an agarose
gel and blotted onto a membrane. The
membrane is hybridized with a probe
designed to complement the target
DNA. Following development of the
signal or image recording, the resulting
banding pattern is analyzed to determine
the genotype or carrier status of
animals within the tested colony.
Western Blotting
We offer
Western Blotting Services for
any protein product. Please
contact customer support for
details on your specific project
needs and custom pricing.
